Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Ryan J. Morris, Marieke Schor, Rachel M.C. Gillespie, Ana Sofia Ferreira, Lucia Baldauf, Chris Earl, Adam Ostrowski, Laura Hobley, Keith M. Bromley, Tetyana Sukhodub, Sofia Arnaouteli, Nicola R. Stanley-Wall, Cait E. MacPhee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

Original languageEnglish
Article number6730
JournalScientific Reports
Issue number1
Publication statusPublished - 27 Jul 2017


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