Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meal as livestock feed

Mulugeta Walelegne, Fentahun Meheret, Melkamu B. Derseh, Mesfin Dejene, Yonas T. Asmare, Prasad KVSV, Chris S. Jones, Robert M. Dixon, Alan Duncan

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Introduction: Measurement of the nutritive value of feedstuffs with near infrared reflectance spectroscopy (NIRS) laboratory benchtop spectrometers is well-established. The aim of this study was to examine the reliability and accuracy of a handheld low-cost Tellspec NIRS spectrometer for measurement of the nutritive value of oilseed meals. Methods: Samples (n=142) comprising byproduct meals from processing linseed, noug seed, cotton seed, groundnut, rapeseed, soybeans, and sunflower seeds were collected from farms, oil factories, wholesalers, and retail shops in central Ethiopia. Samples were scanned ‘as received’ (UGr) and also following drying and grinding (Gr), and were scanned once, twice, and ten times in a 2x3 factorial experimental design. Laboratory analyses of total nitrogen (TN), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and in vitro organic matter digestibility (IVOMD) provided reference measurements. Calibration models were developed using a subset of 2/3 of the spectra and validated using the remaining 1/3 of sample spectra. Results: The sample form and the number of scans, and their interactions, all significantly affected the accuracy of the calibration models (P<0.001). The most accurate calibrations were with Gr samples scanned 10 times, where the coefficient of determination of both calibration and validation sets (R 2cal and R 2val) were ≥ 0.90 for most attributes. The respective standard errors of prediction (SEP) (g/kg DM) for Gr, and ‘as received’, samples respectively that were scanned ten times were: TN (3.2 and 4.7), IVOMD (11.7 and 20.1), NDF (26.9 and 43.8), ADF (25.3 and 44.6), and ADL (6.8 and 10.7). Also, the SEP for each attribute was reduced (P<0.05) by drying and grinding the sample before scanning. The ratios of the standard deviation of the calibration samples to the SEP (RPD) g/kg DM were 2.68, 2.30, 3.17, 3.05, and 4.06 for TN, IVOMD, NDF, ADF, and ADL, respectively, for Gr samples scanned ten times. Nevertheless, the SEP of samples scanned ‘as received’ would often be acceptable for routine analyses in the field and market-place under east African circumstances. Conclusion: In conclusion, NIRS calibrations for a portable handheld Tellspec NIRS spectrometer could be developed to measure important nutritional attributes of oilseed cake samples as feeds.

Original languageEnglish
Article number1203449
Pages (from-to)1-12
Number of pages12
JournalFrontiers in Animal Science
Volume4
Early online date7 Jul 2023
DOIs
Publication statusE-pub ahead of print - 7 Jul 2023

Keywords / Materials (for Non-textual outputs)

  • near infrared (NIR) spectroscopy
  • oilseed
  • Ethiopia
  • Handheld NIRS instrument
  • nutritive value

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