Neo-centromere formation on a 2.6 Mb mini-chromosome in DT40 cells

M H Shen, A Ross, J Yang, J I de las Heras, H Cooke

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes 12 and 15. It is not stable in the CGR8 cells but replicates and segregates with high fidelity after transfer into chicken DT40 cells. Combined analysis by immunocytochemistry/fluorescence in situ hybridisation (FISH) on metaphase spreads detected an active neo-centromere on the mini-chromosome in these cells. Further analysis by immunocytochemistry/FISH on stretched chromatin allowed the localisation of the CENP-C protein to the DNA sequence derived from interval 5 of the human Y chromosome.
Original languageEnglish
Pages (from-to)421-9
Number of pages9
JournalChromosoma
Volume110
Issue number6
DOIs
Publication statusPublished - 2001

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