New applications for phage integrases

Paul C M Fogg; Sean Colloms; Susan Rosser; Marshall Stark; Margaret C M Smith

doi:10.1016/j.jmb.2014.05.014

New applications for phage integrases

Paul C M Fogg, Sean Colloms, Susan Rosser, Marshall Stark, Margaret C M Smith

University of Edinburgh

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from φC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.

Original language	English
Pages (from-to)	2703-2716
Number of pages	14
Journal	Journal of Molecular Biology
Volume	426
Issue number	15
DOIs	https://doi.org/10.1016/j.jmb.2014.05.014
Publication status	Published - 29 Jul 2014

Keywords / Materials (for Non-textual outputs)

bacteriophages
genome engineering
integrases
integrating vectors
synthetic biology

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10.1016/j.jmb.2014.05.014

New applications for phage integrases
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New applications for phage integrases

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Keywords / Materials (for Non-textual outputs)

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