New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields

Liat Adler, Aranzazú Díaz-Ramos, Yuwei Mao, Alistair J McCormick, Krzysztof Robin Pukacz, Chenyi Fei

Research output: Contribution to journalArticlepeer-review

Abstract

Many photosynthetic species have evolved CO2-concentrating mechanisms (CCMs) to improve the efficiency of CO2 assimilation by Rubisco and reduce the negative impacts of photorespiration. However, the majority of plants (i.e. C3 plants) lack an active CCM. Thus, engineering a functional heterologous CCM into important C3 crops, such as rice (Oryza sativa) and wheat (Triticum aestivum), has become a key strategic ambition to enhance yield potential. Here, we review recent advances in our understanding of the pyrenoid-based CCM in the model green alga Chlamydomonas reinhardtii and engineering progress in C3 plants. We also discuss recent modeling work that has provided insights into the potential advantages of Rubisco condensation within the pyrenoid and the energetic costs of the Chlamydomonas CCM, which, together, will help to better guide future engineering approaches. Key findings include the potential benefits of Rubisco condensation for carboxylation efficiency and the need for a diffusional barrier around the pyrenoid matrix. We discuss a minimal set of components for the CCM to function and that active bicarbonate import into the chloroplast stroma may not be necessary for a functional pyrenoid-based CCM in planta. Thus, the roadmap for building a pyrenoid-based CCM into plant chloroplasts to enhance the efficiency of photosynthesis now appears clearer with new challenges and opportunities.

Original languageEnglish
Article numberkiac373
Pages (from-to)1609-1627
Number of pages19
JournalPlant physiology
Volume190
Issue number3
Early online date12 Aug 2022
DOIs
Publication statusPublished - 1 Nov 2022

Keywords

  • algae
  • arabidopsis
  • chlamydomonas
  • liquid-liquid phase separation
  • rubisco
  • photosynthesis

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