New insights into the Manila clam – Perkinsus olseni interaction based on gene expression analysis of clam hemocytes and parasite trophozoites through in vitro challenges

Abdul Farah Md. Hasanuzzamana, Asuncion Cao, Paolo Ronza, Sergio Fernadez Boo, Andres Rubiolo, Diego Robledo, Antonio Gómez-Tato, José Antonio Álvarez-Dios, Belen G Pardo, Antonio Villalba, Paulino Martínez

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Abstract
The Manila clam (Ruditapes philippinarum) is the bivalve species with the highest world production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interaction, we analyzed the gene expression profiles of in vitro challenged clam hemocytes and P. olseni trophozoites, using two oligo-microarray platforms, one previously validated for R. philippinarum hemocytes and a new one developed and validated in this study for P. olseni. Manila clam hemocytes were in vitro challenged with trophozoites, zoospores, and extracellular products from P. olseni in vitro cultures, while P. olseni trophozoites were in vitro challenged with Manila clam plasma along the same time- series (1 h, 8 h, and 24 h). The hemocytes showed a fast activation of the innate immune response, particularly associated with hemocyte recruitment, in the three types of challenges. Nevertheless, different immune-related pathways were activated in response to the different parasite stages, suggesting specific recognition mechanisms. Furthermore, the analyses provided useful complementary data to previous in vivo challenges, and confirmed the potential of some proposed biomarkers. The combined analysis of gene expression in host and parasite identified several processes in both the clam and P. olseni, such as redox and glucose metabolism, protease activity, apoptosis and iron metabolism, whose modulation suggests cross-talk between parasite and host. This information might be critical to determine the outcome of the infection, thus highlighting potential therapeutic targets. Altogether, the results of this study aid to understand the response and interaction between R. philippinarum–P. olseni and will contribute for developing effective control strategies for this threatening parasitosis.
Original languageEnglish
JournalInternational Journal For Parasitology
Early online date20 Feb 2020
DOIs
Publication statusE-pub ahead of print - 20 Feb 2020

Keywords / Materials (for Non-textual outputs)

  • Manila clam
  • perkinsosis
  • in vitro challenge
  • gene expression
  • hemocytes
  • host-parasite interaction

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