Next-generation analysis of trypanosomatid genome stability and instability

Emma M. Briggs, Catarina A. Marques, Joao Reis-Cunha, Jennifer Black, Samantha Campbell, Jeziel Damasceno, Daniella Bartholomeu, Kathryn Crouch, Richard McCulloch*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

Understanding the rate and patterns of genome variation is becoming ever more amenable to whole-genome analysis through advances in DNA sequencing, which may, at least in some circumstances, have supplanted more localized analyses by cellular and genetic approaches. Whole-genome analyses can utilize both short- and long-read sequence technologies. Here we describe how sequence generated by these approaches has been used in trypanosomatids to examine DNA replication dynamics, the accumulation of modified histone H2A due to genome damage, and evaluation of genome variation, focusing on ploidy change.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHUMANA PRESS INC
Pages225-262
Number of pages38
ISBN (Electronic)978-1-0716-0294-2
ISBN (Print)978-1-0716-0293-5
DOIs
Publication statusE-pub ahead of print - 28 Mar 2020

Publication series

NameMethods in Molecular Biology
Volume2116
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords / Materials (for Non-textual outputs)

  • ChIP-seq
  • copy number variation
  • DNA damage
  • DNA replication
  • MFA-seq
  • next-generation sequencing
  • ploidy
  • single nucleotide polymorphisms

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