Projects per year
Abstract
Nuclear factors NF90 and NF45 form a complex involved in a variety of cellular processes and are thought to affect gene expression both at the transcriptional and translational level. In addition, this complex affects the replication of several viruses through direct interactions with viral RNA. NF90 and NF45 dimerize through their common 'DZF' domain (domain associated with zinc fingers). NF90 has additional double-stranded RNA-binding domains that likely mediate its association with target RNAs. We present the crystal structure of the NF90/NF45 dimerization complex at 1.9-Å resolution. The DZF domain shows structural similarity to the template-free nucleotidyltransferase family of RNA modifying enzymes. However, both NF90 and NF45 have lost critical catalytic residues during evolution and are therefore not functional enzymes. Residues on NF90 that make up its interface with NF45 are conserved in two related proteins, spermatid perinuclear RNA-binding protein (SPNR) and zinc-finger RNA-binding protein (Zfr). Using a co-immunoprecipitation assay and site-specific mutants, we demonstrate that NF45 is also able to recognize SPNR and Zfr through the same binding interface, revealing that NF45 is able to form a variety of cellular complexes with other DZF-domain proteins.
Original language | English |
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Pages (from-to) | 9356-9368 |
Journal | Nucleic Acids Research |
Volume | 40 |
Issue number | 18 |
DOIs | |
Publication status | Published - 2012 |
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Dive into the research topics of 'NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold'. Together they form a unique fingerprint.Projects
- 3 Finished
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Core funding renewal for the Wellcome Trust Centre for Cell Biology
1/10/11 → 30/04/17
Project: Research
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Murnau
Atlanta Cook (Speaker)
17 Oct 2012 → 20 Oct 2012Activity: Participating in or organising an event types › Participation in conference
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MPI Developmental Biology, Tuebingen, external seminar
Atlanta Cook (Speaker)
12 Oct 2012Activity: Academic talk or presentation types › Invited talk
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complex life of mRNA
Atlanta Cook (Participant)
7 Oct 2012 → 10 Oct 2012Activity: Participating in or organising an event types › Participation in conference