Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step

Manish Gupta, Anne Caniard, Angeles Touceda-Varela, Dominic J Campopiano, Juan C Mareque-Rivas

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate that QDs coated with nitrilotriacetic acid (NTA) bound to Ni (2+) can be used to reversibly and selectively bind, purify, and fluorescently label His 6-tagged (N-terminal) glutathione S-transferase (GST) in one step with retention of enzymatic activity. We find binding to be less effective in the absence of the His 6-tag or Ni (2+) ions.
Original languageEnglish
Pages (from-to)1964-7
Number of pages4
JournalBioconjugate chemistry
Volume19
Issue number10
DOIs
Publication statusPublished - 2008

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