Abstract
We demonstrate that QDs coated with nitrilotriacetic acid (NTA) bound to Ni (2+) can be used to reversibly and selectively bind, purify, and fluorescently label His 6-tagged (N-terminal) glutathione S-transferase (GST) in one step with retention of enzymatic activity. We find binding to be less effective in the absence of the His 6-tag or Ni (2+) ions.
Original language | English |
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Pages (from-to) | 1964-7 |
Number of pages | 4 |
Journal | Bioconjugate chemistry |
Volume | 19 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2008 |