NK-like CD8(+) cells in immunologically naïve neonatal calves that respond to dendritic cells infected with Mycobacterium bovis BCG

Jayne Hope, Paul Sopp, Chris J Howard

Research output: Contribution to journalArticlepeer-review

Abstract

Pre-exposure to environmental mycobacteria and induction of an inappropriately biased immune response may be major factors affecting the efficacy of BCG; vaccination of neonates that have not been exposed to environmental mycobacteria may induce more effective immunity. Responses of neonatal calves to mycobacterial antigens using dendritic cells (DC) as antigen-presenting cells were investigated. In nonvaccinated, immunologically naive calves as young as 1 day old, a population of CD8(+) cells proliferated and produced IFN-gamma in response to BCG-infected DC. CD3(-) CD8(+) NK-like and CD3(+) CD8(+) T cells were evident within the responding CD8(+) population. The response was not MHC-restricted. The NK-like CD3(-) cells were the major population producing IFN-gamma. The presence of mycobacteria-reactive, IFN-gamma-secreting CD8(+) NK cells in neonatal calves may have important consequences for the induction of a Th1-biased immune response.
Original languageEnglish
Pages (from-to)184-94
Number of pages11
JournalJournal of Leukocyte Biology
Volume71
Issue number2
Publication statusPublished - Feb 2002

Keywords

  • Animals
  • Animals, Newborn
  • Antigen Presentation
  • Antigens, CD8
  • BCG Vaccine
  • Cattle
  • Cytotoxicity, Immunologic
  • Dendritic Cells/immunology
  • Immunity
  • Killer Cells, Natural/immunology
  • Mycobacterium bovis/immunology
  • Th1 Cells/immunology
  • Tuberculosis, Bovine/immunology
  • Tuberculosis, Bovine/prevention & control

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