Projects per year
Abstract
The NKp46 receptor demonstrates a high degree of lineage specificity, being expressed almost exclusively in NK cells. Previous studies have demonstrated NKp46 expression by T cells, but NKp46(+)CD3(+) cells are rare and almost universally associated with NKp46 acquisition by T cells following stimulation. In this study we demonstrate the existence of a population of NKp46(+)CD3(+) cells resident in normal bovine PBMCs that includes cells of both the αβ TCR(+) and γδ TCR(+) lineages and is present at a frequency of 0.1-1.7%. NKp46(+)CD3(+) cells express transcripts for a broad repertoire of both NKRs and TCRs and also the CD3ζ, DAP10, and FcεR1γ but not DAP12 adaptor proteins. In vitro functional analysis of NKp46(+)CD3(+) cells confirm that NKp46, CD16, and CD3 signaling pathways are all functionally competent and capable of mediating/redirecting cytolysis. However, only CD3 cross-ligation elicits IFN-γ release. NKp46(+)CD3(+) cells exhibit cytotoxic activity against autologous Theileria parva-infected cells in vitro, and during in vivo challenge with this parasite an expansion of NKp46(+)CD3(+) cells was observed in some animals, indicating the cells have the potential to act as an anti-pathogen effector population. The results in this study identify and describe a novel nonconventional NKp46(+)CD3(+) T cell subset that is phenotypically and functionally distinct from conventional NK and T cells. The ability to exploit both NKRs and TCRs suggests these cells may fill a functional niche at the interface of innate and adaptive immune responses.
Original language | English |
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Pages (from-to) | 3868-3880 |
Number of pages | 13 |
Journal | Journal of Immunology |
Volume | 192 |
Issue number | 8 |
DOIs | |
Publication status | Published - 15 Apr 2014 |
Keywords
- NATURAL-KILLER-CELLS
- PRODUCE GAMMA-INTERFERON
- RECEPTOR-ALPHA-CHAIN
- THEILERIA-PARVA
- NEOSPORA-CANINUM
- MONOCLONAL-ANTIBODIES
- CD94-NKG2A RECEPTORS
- MYCOBACTERIUM-BOVIS
- CELIAC-DISEASE
- NKP46 DEFINES
Fingerprint
Dive into the research topics of 'NKp46+CD3+ Cells: A Novel Nonconventional T Cell Subsetin Cattle Exhibiting Both NK Cell and T Cell Features'. Together they form a unique fingerprint.Projects
- 2 Finished
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Innate immunity and endemic diseases in livestock species
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
1/04/12 → 31/03/17
Project: Research
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Uderstanding the basis of strain-restricted immunity to theileria parva
Morrison, I.
1/03/10 → 31/08/13
Project: Research
Profiles
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Tim Connelley
- Royal (Dick) School of Veterinary Studies - Group Leader
Person: Academic: Research Active
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Jayne Hope
- Royal (Dick) School of Veterinary Studies - Personal Chair of Immunology
- Edinburgh Imaging
Person: Academic: Research Active