Nob1p is required for cleavage of the 3 ' end of 18S rRNA

A Fatica, M Oeffinger, M Dlakic, D Tollervey

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We report the characterization of a novel factor, Nob1p (Yor056c), which is essential for the synthesis of 40S ribosome subunits. Genetic depletion of Nob1p strongly inhibits the processing of the 20S pre-rRNA to the mature 18S rRNA, leading to the accumulation of high levels of the 20S pre-rRNA together with novel degradation intermediates. 20S processing occurs within a pre-40S particle after its export from the nucleus to the cytoplasm. Consistent with a direct role in this cleavage, Nob1p was shown to be associated with the pre-40S particle and to be present in both the nucleus and the cytoplasm. This suggests that Nob1p accompanies the pre-40S ribosomes during nuclear export. Pre-40S export is not, however, inhibited by depletion of Nob1p.

Original languageEnglish
Pages (from-to)1798-1807
Number of pages10
JournalMolecular and Cellular Biology
Volume23
Issue number5
DOIs
Publication statusPublished - Mar 2003

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