Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library.

Alexander Gosta Ekstrom; Jue Theresa Wang; Juraj Bella; Dominic James Campopiano

doi:10.1039/C8OB01918E

Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library.

Alexander Gosta Ekstrom, Jue Theresa Wang, Juraj Bella, Dominic James Campopiano

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used 19F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAHs). An NAH DCL, constructed from a fluoro-aromatic aldehyde and a small set of hydrazides, was targetted at ecFabH, an essential enzyme in bacterial fatty acid biosynthesis. Our NMR analysis identified a tert-butyl NAH as the best binder which was confirmed by enzymatic assay.

Original language	English
Journal	Organic & Biomolecular chemistry
Early online date	4 Oct 2018
DOIs	https://doi.org/10.1039/C8OB01918E
Publication status	E-pub ahead of print - 4 Oct 2018

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10.1039/C8OB01918E

20181004 Campopiano Ekstrom et al OBCAccepted author manuscript, 1.31 MBLicence: Creative Commons: Attribution (CC-BY)

http://pubs.rsc.org/en/Content/ArticleLanding/2018/OB/C8OB01918E

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title = "Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library.",

abstract = "Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used 19F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAHs). An NAH DCL, constructed from a fluoro-aromatic aldehyde and a small set of hydrazides, was targetted at ecFabH, an essential enzyme in bacterial fatty acid biosynthesis. Our NMR analysis identified a tert-butyl NAH as the best binder which was confirmed by enzymatic assay. ",

author = "Ekstrom, {Alexander Gosta} and Wang, {Jue Theresa} and Juraj Bella and Campopiano, {Dominic James}",

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AU - Wang, Jue Theresa

AU - Bella, Juraj

AU - Campopiano, Dominic James

PY - 2018/10/4

Y1 - 2018/10/4

N2 - Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used 19F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAHs). An NAH DCL, constructed from a fluoro-aromatic aldehyde and a small set of hydrazides, was targetted at ecFabH, an essential enzyme in bacterial fatty acid biosynthesis. Our NMR analysis identified a tert-butyl NAH as the best binder which was confirmed by enzymatic assay.

AB - Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used 19F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAHs). An NAH DCL, constructed from a fluoro-aromatic aldehyde and a small set of hydrazides, was targetted at ecFabH, an essential enzyme in bacterial fatty acid biosynthesis. Our NMR analysis identified a tert-butyl NAH as the best binder which was confirmed by enzymatic assay.

U2 - 10.1039/C8OB01918E

DO - 10.1039/C8OB01918E

M3 - Article

SN - 1477-0520

JO - Organic & Biomolecular chemistry

JF - Organic & Biomolecular chemistry

ER -

Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library.

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