Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes

R. Mouras; P. Bagnaninchi; A. Downes; M. Muratore; A. Elfick

doi:10.1117/12.889780

Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes

R. Mouras, P. Bagnaninchi, A. Downes, M. Muratore, A. Elfick

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Adipose-derived stem cells (ADSCs) are adult stem cells isolated from lipoaspirates. They are a good candidate for autologuous cell therapy and tissue engineering. For these applications, label-free imaging could be critical to assess noninvasively the efficiency of stem cell (SC) differentiation. We report on the development and application of a multimodal microscope to monitor and quantify ADSC differentiation into osteoblasts and adipocytes.

Original language	English
Title of host publication	ADVANCED MICROSCOPY TECHNIQUES II
Editors	PTC So, E Beaurepaire
Place of Publication	BELLINGHAM
Publisher	SPIE
Pages	-
Number of pages	7
ISBN (Print)	978-0-81948-683-7
DOIs	https://doi.org/10.1117/12.889780
Publication status	Published - 2011
Event	Conference on Advanced Microscopy Techniques II - Munich Duration: 22 May 2011 → 24 May 2011

Conference

Conference	Conference on Advanced Microscopy Techniques II
City	Munich
Period	22/05/11 → 24/05/11

Keywords / Materials (for Non-textual outputs)

NLO
Multiphoton microscopy
CARS
SHG
live cell imaging
Stem cells
Osteoblasts
Adipocytes
STOKES-RAMAN SCATTERING
TISSUE

Access to Document

10.1117/12.889780

Cite this

@inproceedings{d01f7a319e0046168269ebcac56e3a8d,

title = "Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes",

abstract = "Adipose-derived stem cells (ADSCs) are adult stem cells isolated from lipoaspirates. They are a good candidate for autologuous cell therapy and tissue engineering. For these applications, label-free imaging could be critical to assess noninvasively the efficiency of stem cell (SC) differentiation. We report on the development and application of a multimodal microscope to monitor and quantify ADSC differentiation into osteoblasts and adipocytes.",

keywords = "NLO, Multiphoton microscopy, CARS, SHG, live cell imaging, Stem cells, Osteoblasts, Adipocytes, STOKES-RAMAN SCATTERING, TISSUE",

author = "R. Mouras and P. Bagnaninchi and A. Downes and M. Muratore and A. Elfick",

year = "2011",

doi = "10.1117/12.889780",

language = "English",

isbn = "978-0-81948-683-7",

pages = "--",

editor = "PTC So and E Beaurepaire",

booktitle = "ADVANCED MICROSCOPY TECHNIQUES II",

publisher = "SPIE",

address = "United States",

note = "Conference on Advanced Microscopy Techniques II ; Conference date: 22-05-2011 Through 24-05-2011",

}

TY - GEN

T1 - Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes

AU - Mouras, R.

AU - Bagnaninchi, P.

AU - Downes, A.

AU - Muratore, M.

AU - Elfick, A.

PY - 2011

Y1 - 2011

N2 - Adipose-derived stem cells (ADSCs) are adult stem cells isolated from lipoaspirates. They are a good candidate for autologuous cell therapy and tissue engineering. For these applications, label-free imaging could be critical to assess noninvasively the efficiency of stem cell (SC) differentiation. We report on the development and application of a multimodal microscope to monitor and quantify ADSC differentiation into osteoblasts and adipocytes.

AB - Adipose-derived stem cells (ADSCs) are adult stem cells isolated from lipoaspirates. They are a good candidate for autologuous cell therapy and tissue engineering. For these applications, label-free imaging could be critical to assess noninvasively the efficiency of stem cell (SC) differentiation. We report on the development and application of a multimodal microscope to monitor and quantify ADSC differentiation into osteoblasts and adipocytes.

KW - NLO

KW - Multiphoton microscopy

KW - CARS

KW - SHG

KW - live cell imaging

KW - Stem cells

KW - Osteoblasts

KW - Adipocytes

KW - STOKES-RAMAN SCATTERING

KW - TISSUE

UR - http://www.scopus.com/inward/record.url?scp=79960549006&partnerID=8YFLogxK

U2 - 10.1117/12.889780

DO - 10.1117/12.889780

M3 - Conference contribution

SN - 978-0-81948-683-7

SP - -

BT - ADVANCED MICROSCOPY TECHNIQUES II

A2 - So, PTC

A2 - Beaurepaire, E

PB - SPIE

CY - BELLINGHAM

T2 - Conference on Advanced Microscopy Techniques II

Y2 - 22 May 2011 through 24 May 2011

ER -

Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes

Abstract

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

A MULTI MODAL TWO PHOTON/CARS IMAGING SYSTEM

RCUK Fellowship

Cite this

Non-Linear Optical Microscopy of Adipose-Derived Stem Cells Induced Towards Osteoblasts and Adipocytes

Abstract

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

A MULTI MODAL TWO PHOTON/CARS IMAGING SYSTEM

RCUK Fellowship

Cite this