Abstract
Some opioid-resistant pain conditions can be alleviated by voltage-dependent Na(+) channel blockers such as lamotrigine. The mu-opioid-receptor agonist morphine can modulate cation entry into cells to affect overall cellular excitability, an effect which can in turn be endogenously antagonised by the neuropeptide cholecystokinin (CCK). However, lamotrigine may also modulate cellular excitability by non-specifically blocking voltage-dependent ion channels. We have looked for interactions of lamotrigine with the opioid/CCK pathway within the spinal dorsal horn, to rule out the possibility that lamotrigine may attenuate nociceptive responses via actions on this pathway. Both lamotrigine and the mu-opioid agonist DAMGO inhibited mustard oil-evoked cell firing by approximately 50% compared with control levels. Co-application of CCK8S reversed DAMGO-, but not lamotrigine-induced inhibition of cell firing and this reversal was prevented with the selective CCK(B) receptor antagonist PD 135158. Although lamotrigine inhibited both brush- and cold-evoked cell firing in neuropathic animals, lamotrigine inhibition of mustard oil-evoked cell firing in the same animals was not significantly greater than that observed in controls. These results suggest that the antinociceptive properties of lamotrigine within the spinal dorsal horn are unlikely to be mediated via interactions with the opioid/CCK pathway.
Original language | English |
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Pages (from-to) | 385-90 |
Number of pages | 6 |
Journal | Neuroscience Research |
Volume | 39 |
Issue number | 4 |
Publication status | Published - Apr 2001 |
Keywords / Materials (for Non-textual outputs)
- Action Potentials
- Analgesics, Opioid
- Animals
- Anti-Anxiety Agents
- Calcium Channel Blockers
- Cholecystokinin
- Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
- Indoles
- Inflammation
- Male
- Meglumine
- Mustard Plant
- Neural Pathways
- Nociceptors
- Opioid Peptides
- Pain
- Peripheral Nervous System Diseases
- Plant Extracts
- Plant Oils
- Posterior Horn Cells
- Rats
- Rats, Wistar
- Sincalide
- Triazines