Non-typeable Haemophilus influenzae infection of the Junbo mouse

Derek Hood, Michael Cheeseman

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Acute otitis media, inflammation of the middle ear bulla, is the most common bacterial infection in children. For one of the principal otopathogens, non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate host-microbial interactions relevant to the onset and progression of infection and study treatment of middle ear disease. We have established a robust model of NTHi middle ear infection in the Junbo mouse. Intranasal inoculation with NTHi produces high rates of bulla infection and bacterial titres in bulla fluids; bacteria can also spread down the respiratory tract to the mouse lung. An innate immune response is detected in the bulla of Junbo mice following NTHi infection and bacteria are maintained in some ears at least up to day 56 post-inoculation. The Junbo/NTHi infection model facilitates studies on bacterial pathogenesis and antimicrobial intervention regimens and vaccines for better treatment and prevention of NTHi middle ear infection.
Original languageEnglish
Pages (from-to)29-46
Number of pages18
JournalCurrent Protocols in Mouse Biology
Volume7
Issue number1
Early online date2 Mar 2017
DOIs
Publication statusE-pub ahead of print - 2 Mar 2017

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