Nonamplifying alleles at microsatellite loci - a caution for parentage and population studies

J M  PEMBERTON; J  SLATE; D R  BANCROFT; J A  BARRETT; Josephine Pemberton

Nonamplifying alleles at microsatellite loci - a caution for parentage and population studies

J M PEMBERTON, J SLATE, D R BANCROFT, J A BARRETT, Josephine Pemberton

School of Biological Sciences

Research output: Contribution to journal › Comment/debate › peer-review

Abstract / Description of output

While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and Ce1JP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-off spring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.

Original language	English
Pages (from-to)	249-252
Number of pages	4
Journal	Molecular Ecology
Volume	4
Issue number	2
Publication status	Published - Apr 1995

Cite this

@article{5c792aa2eac6487f9f2c534394e049e2,

title = "Nonamplifying alleles at microsatellite loci - a caution for parentage and population studies",

abstract = "While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and Ce1JP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-off spring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.",

author = "PEMBERTON, {J M} and J SLATE and BANCROFT, {D R} and BARRETT, {J A} and Josephine Pemberton",

year = "1995",

month = apr,

language = "English",

volume = "4",

pages = "249--252",

journal = "Molecular Ecology",

issn = "0962-1083",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Nonamplifying alleles at microsatellite loci - a caution for parentage and population studies

AU - PEMBERTON, J M

AU - SLATE, J

AU - BANCROFT, D R

AU - BARRETT, J A

AU - Pemberton, Josephine

PY - 1995/4

Y1 - 1995/4

N2 - While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and Ce1JP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-off spring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.

AB - While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and Ce1JP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-off spring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.

M3 - Comment/debate

SN - 0962-1083

VL - 4

SP - 249

EP - 252

JO - Molecular Ecology

JF - Molecular Ecology

IS - 2

ER -

Nonamplifying alleles at microsatellite loci - a caution for parentage and population studies

Abstract / Description of output

Fingerprint

Cite this