Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with Tc-99m-HMPAO CBF autoradiography in a rat stroke model

Tracey A. Baskerville, Christopher McCabe, Christopher J. Weir, I. Mhairi Macrae*, William M. Holmes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Arterial spin labelling (ASL) is increasingly available for noninvasive cerebral blood flow (CBF) measurement in stroke research. Here, a pseudo-continuous ASL technique (pCASL) was evaluated against Tc-99m-D, L-hexamethylpropyleneamine oxime (Tc-99m-HMPAO) autoradiography in a rat stroke model. The Tc-99m-HMPAO was injected (intravenously, 225 MBq) during pCASL acquisition. The pCASL and Tc-99m-HMPAO autoradiography CBF measures, relative to the contralateral hemisphere, were in good agreement across the spectrum of flow values in normal and ischemic tissues. The pCASL-derived quantitative regional CBF values (contralateral: 157 to 177 mL/100 g per minute; ipsilateral: 9 to 104 mL/100 g per minute) were consistent with the literature values. The data show the potential utility of pCASL for CBF assessment in a rat stroke model. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 973-977; doi:10.1038/jcbfm.2012.19; published online 4 April 2012

Original languageEnglish
Pages (from-to)973-977
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume32
Issue number6
DOIs
Publication statusPublished - Jun 2012

Keywords

  • stroke
  • OCCLUSION
  • CEREBRAL-BLOOD-FLOW
  • BRAIN
  • INVERSION
  • PERFUSION
  • ASL
  • CBF
  • FOCAL ISCHEMIA

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