NONSENSE-MEDIATED MRNA DECAY IN CAENORHABDITIS ELEGANS

Dasa Longman, Pablo Arrisi, Iain L. Johnstone, Javier F. Caceres

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Abstract

The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.

Original languageEnglish
Title of host publicationRNA TURNOVER IN EUKARYOTES: ANALYSIS OF SPECIALIZED AND QUALITY CONTROL RNA DECAY PATHWAYS
Place of PublicationSAN DIEGO
PublisherELSEVIER ACADEMIC PRESS INC
Pages149-164
Number of pages16
DOIs
Publication statusPublished - 2008

Cite this