Abstract / Description of output
The normal PrP(C) (cellular prion protein) contains sLe(X) [sialyl-Le(X) (Lewis X)] and Le(X). sLe(X) is a ligand of selectins. To examine whether PrP(C) is a ligand of selectins, we generated three human PrP(C)-Ig fusion proteins: one with Le(X), one with sLe(X), and the other with neither Le(X) nor sLe(X). Only Le(X)-PrP(C)-Ig binds E-, L- and P-selectins. Binding is Ca(2+)-dependent and occurs with nanomolar affinity. Removal of sialic acid on sLe(X)-PrP(C)-Ig enables the fusion protein to bind all selectins. These findings were confirmed with brain-derived PrP(C). The selectins precipitated PrP(C) in human brain in a Ca(2+)-dependent manner. Treatment of brain homogenates with neuraminidase increased the amounts of PrP(C) precipitated. Therefore the presence of sialic acid prevents the binding of PrP(C) in human brain to selectins. Hence, human brain PrP(C) interacts with selectins in a manner that is distinct from interactions in peripheral tissues. Alternations in these interactions may have pathological consequences.
Original language | English |
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Pages (from-to) | 333-41 |
Number of pages | 9 |
Journal | Biochemical Journal |
Volume | 406 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Sept 2007 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Antigens, CD15
- Antigens, CD24
- Brain
- Calcium
- Cell Line
- Epitopes
- Gene Expression Regulation
- Humans
- Immunoglobulin G
- Lewis Blood-Group System
- Ligands
- Mice
- Neuraminidase
- Oligosaccharides
- Polysaccharides
- Prions
- Protein Binding
- Recombinant Fusion Proteins
- Selectins