Novel atypical Aeromonas salmonicida bath challenge model for juvenile ballan wrasse (Labrus bergylta, Ascanius)

Athina Papadopoulou, Kathryn Garvey, Tom Hill , J. G. Ramirez Paredes, Sean J. Monaghan, Johanna Baily, Andrew Davie, Ioanna Katsiadaki, D. W. Verner-Jeffreys, Timothy S. Wallis, Hervé Migaud, Alexandra Adams

Research output: Contribution to journalArticlepeer-review

Abstract

Atypical Aeromonas salmonicida (aAs) is currently one of the most routinely recovered bacterial pathogens isolated during disease outbreaks in farmed cleaner fish, ballan wrasse (Labrus bergylta, Ascanius). Vibrionaceae family bacteria have also been isolated from ballan wrasse in Scotland. This study determined the infectivity, pathogenicity and virulence of aAs and Vibrionaceae isolates in juvenile farmed ballan wrasse (n= 50; approx. 2 gr) using a bath challenge and fish monitored for a period of 16 days. Atypical As caused significant mortalities in contrast to Vibrionaceae isolates. Notably, differential virulence was observed between two aAs vapA type V strains at similar challenge doses. Diseased fish exhibited a systemic infection where aAs was detected in all analysed tissues (liver, spleen and kidney) by PCR and qPCR. Macroscopically, moribund and survivor fish exhibited hepatomegaly and splenomegaly. In moribund and surviving fish histopathology showed granulomatous hepatitis
with eosinophilic granular cells surrounding bacterial colonies and endocarditis along with splenic histiocytosis. This is the first report of a successful aAs bath challenge model for juvenile ballan wrasse which provides an important tool for future studies on vaccine efficacy and immunocompetence.

Original languageEnglish
JournalJournal of Fish Diseases
DOIs
Publication statusPublished - 4 Dec 2020

Keywords

  • atypical Aeromonas salmonicida
  • bath challenge
  • gross pathology
  • systemic 40 disease
  • eosinophilic granular cells (EGCs)

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