Novel biochip platform for nucleic acid analysis

Salvatore Pernagallo; Giorgio Ventimiglia; Claudia Cavalluzzo; Enrico Alessi; Hugh Ilyine; Mark Bradley; Juan J Diaz-Mochon

doi:10.3390/s120608100

Novel biochip platform for nucleic acid analysis

Salvatore Pernagallo, Giorgio Ventimiglia, Claudia Cavalluzzo, Enrico Alessi, Hugh Ilyine, Mark Bradley, Juan J Diaz-Mochon

Research output: Contribution to journal › Article › peer-review

Abstract

This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top "footprint" requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

Original language	English
Pages (from-to)	8100-11
Number of pages	12
Journal	Sensors
Volume	12
Issue number	6
DOIs	https://doi.org/10.3390/s120608100
Publication status	Published - 2012

Keywords / Materials (for Non-textual outputs)

Base Sequence
Genomics
Humans
Mengovirus
MicroRNAs
Microfluidic Analytical Techniques
Molecular Sequence Data
Nucleic Acid Probes
Nucleic Acids
Oligonucleotides
RNA, Viral
Reproducibility of Results

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Cite this

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AU - Pernagallo, Salvatore

AU - Ventimiglia, Giorgio

AU - Cavalluzzo, Claudia

AU - Alessi, Enrico

AU - Ilyine, Hugh

AU - Bradley, Mark

AU - Diaz-Mochon, Juan J

PY - 2012

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AB - This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top "footprint" requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

KW - Base Sequence

KW - Genomics

KW - Humans

KW - Mengovirus

KW - MicroRNAs

KW - Microfluidic Analytical Techniques

KW - Molecular Sequence Data

KW - Nucleic Acid Probes

KW - Nucleic Acids

KW - Oligonucleotides

KW - RNA, Viral

KW - Reproducibility of Results

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JO - Sensors

JF - Sensors

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Novel biochip platform for nucleic acid analysis

Abstract

Keywords / Materials (for Non-textual outputs)

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