Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice

Yuhai Bi, Lu Lu, Jing Li, Yanbo Yin, Yi Zhang, Huijie Gao, Zhuoming Qin, Basit Zeshan, Jinhua Liu, Lei Sun, Wenjun Liu

Research output: Contribution to journalArticlepeer-review


BACKGROUND: H9N2 influenza A viruses have undergone extensive reassortments in different host species, and could lead to the epidemics or pandemics with the potential emergence of novel viruses.

METHODS: To understand the genetic and pathogenic features of early and current circulating H9N2 viruses, 15 representative H9N2 viruses isolated from diseased chickens in northern China between 1998 and 2010 were characterized and compared with all Chinese H9N2 viruses available in the NCBI database. Then, the representative viruses of different genotypes were selected to study the pathogenicity in mice with the aim to investigate the adaptation and the potential pathogenicity of the novel H9N2 reassortants to mammals.

RESULTS: Our results demonstrated that most of the 15 isolates were reassortants and generated four novel genotypes (B62-B65), which incorporated the gene segments from Eurasian H9N2 lineage, North American H9N2 branch, and H5N1 viruses. It was noteworthy that the newly identified genotype B65 has been prevalent in China since 2007, and more importantly, different H9N2 influenza viruses displayed a diverse pathogenicity to mice. The isolates of the 2008-2010 epidemic (genotypes B55 and B65) were lowly infectious, while two representative viruses of genotypes B0 and G2 isolated from the late 1990s were highly pathogenic to mice. In addition, Ck/SD/LY-1/08 (genotype 63, containing H5N1-like NP and PA genes) was able to replicate well in mouse lungs with high virus titers but caused mild clinical signs.

CONCLUSION: Several lines of evidence indicated that the H9N2 influenza viruses constantly change their genetics and pathogenicity. Thus, the genetic evolution of H9N2 viruses and their pathogenicity to mammals should be closely monitored to prevent the emergence of novel pandemic viruses.

Original languageEnglish
Pages (from-to)505
JournalVirology Journal
Publication statusPublished - 4 Nov 2011


  • Animals
  • Chickens
  • China
  • Cluster Analysis
  • Disease Models, Animal
  • Evolution, Molecular
  • Female
  • Influenza A Virus, H9N2 Subtype
  • Influenza in Birds
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Orthomyxoviridae Infections
  • Phylogeny
  • RNA, Viral
  • Reassortant Viruses
  • Sequence Analysis, DNA
  • Journal Article
  • Research Support, Non-U.S. Gov't


Dive into the research topics of 'Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice'. Together they form a unique fingerprint.

Cite this