Novel microRNAs encoded by duck enteritis virus

Y. X. Yao; L. P. Smith; L. Petherbridge; M. Watson; V. Nair

doi:10.1099/vir.0.040634-0

Novel microRNAs encoded by duck enteritis virus

Y. X. Yao, L. P. Smith, L. Petherbridge, M. Watson, V. Nair

Research output: Contribution to journal › Article › peer-review

Abstract

Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.

Original language	English
Pages (from-to)	1530-1536
Number of pages	7
Journal	Journal of General Virology
Volume	93
Issue number	7
DOIs	https://doi.org/10.1099/vir.0.040634-0
Publication status	Published - 2012

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10.1099/vir.0.040634-0

http://vir.sgmjournals.org/content/93/Pt_7/1530

ISP4 Pathogenesis and resistance in viral diseases of livestock
Dutia, B., Archibald, A., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Collie, D., Digard, P., Freeman, T., Glass, E., Grey, F., Hocking, P., Houston, R., Hume, D., Kaiser, P., Nash, A., Sang, H., Sharp, C., Watson, M. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research
Ark Genomics
Watson, M., Archibald, A. & Burt, D.
BBSRC
1/04/12 → 31/03/17
Project: Research
Defining the cistrome and quantitative transcriptome of virus-transformed cells using massively parallell sequencing
Watson, M.
BBSRC
4/11/11 → 1/04/15
Project: Research

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title = "Novel microRNAs encoded by duck enteritis virus",

abstract = "Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.",

author = "Yao, {Y. X.} and Smith, {L. P.} and L. Petherbridge and M. Watson and V. Nair",

note = "Times Cited: 0 Yao, Yongxiu Smith, Lorraine P. Petherbridge, Lawrence Watson, Mick Nair, Venugopal Part 7",

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doi = "10.1099/vir.0.040634-0",

language = "English",

volume = "93",

pages = "1530--1536",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Society for General Microbiology ",

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TY - JOUR

T1 - Novel microRNAs encoded by duck enteritis virus

AU - Yao, Y. X.

AU - Smith, L. P.

AU - Petherbridge, L.

AU - Watson, M.

AU - Nair, V.

N1 - Times Cited: 0 Yao, Yongxiu Smith, Lorraine P. Petherbridge, Lawrence Watson, Mick Nair, Venugopal Part 7

PY - 2012

Y1 - 2012

N2 - Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.

AB - Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.

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DO - 10.1099/vir.0.040634-0

M3 - Article

VL - 93

SP - 1530

EP - 1536

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -

Novel microRNAs encoded by duck enteritis virus

Abstract

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Projects

ISP4 Pathogenesis and resistance in viral diseases of livestock

Ark Genomics

Defining the cistrome and quantitative transcriptome of virus-transformed cells using massively parallell sequencing

Cite this