Projects per year
Abstract / Description of output
A variety of spindle and kinetochore defects have been shown to induce a mitotic delay through activation of the spindle checkpoint. With the aim of identifying novel mitotic defects we carried out a mad1 synthetic lethal screen in budding yeast. In this screen, four novel alleles of sfi1 were isolated. SFI1 is an essential gene, previously identified through its interaction with centrin/CDC31 and shown to be required for spindle pole body (SPB) duplication. The new mutations were all found in the C-terminal domain of Sfi1p, which has no known function, but it is well conserved among budding yeasts. Analysis of the novel sfi1 mutants, through a combination of light and electron microscopy, revealed duplicated SPBs
FingerprintDive into the research topics of 'Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function'. Together they form a unique fingerprint.
- 1 Finished
1/10/02 → 30/09/05