Nuclear factor I is specifically targeted to discrete subnuclear sites in adenovirus type 2-infected cells

J Bosher, A Dawson, R T Hay

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

During the S phase of the eukaryotic cell cycle and in virus-infected cells, DNA replication takes place at discrete sites in the nucleus, although it is not clear how the proteins involved in the replicative process are directed to these sites. Nuclear factor I is a cellular, sequence-specific DNA-binding protein utilized by adenovirus type 2 to facilitate the assembly of a nucleoprotein complex at the viral origin of DNA replication. Immunofluorescence experiments reveal that in uninfected cells, nuclear factor I is distributed evenly throughout the nucleus. However, after a cell is infected with adenovirus type 2, the distribution of nuclear factor I is dramatically altered, being colocalized with the viral DNA-binding protein in a limited number of subnuclear sites which bromodeoxyuridine pulse-labeling experiments have identified as sites of viral DNA replication. Experiments with adenovirus type 4, which does not require nuclear factor I for viral DNA replication, indicate that although the adenovirus type 4 DNA-binding protein is localized to discrete nuclear sites, this does not result in the redistribution of nuclear factor I. Localization of nuclear factor I to discrete subnuclear sites is therefore likely to represent a specific targeting event that reflects the requirement for nuclear factor I in adenovirus type 2 DNA replication.

Original languageEnglish
Pages (from-to)3140-50
Number of pages11
JournalJournal of Virology
Issue number5
Publication statusPublished - May 1992

Keywords / Materials (for Non-textual outputs)

  • Adenovirus Infections, Human/metabolism
  • Adenoviruses, Human/metabolism
  • CCAAT-Enhancer-Binding Proteins
  • Cell Nucleus/chemistry
  • DNA Replication
  • DNA, Viral/metabolism
  • DNA-Binding Proteins/isolation & purification
  • Host-Parasite Interactions
  • Humans
  • Immunohistochemistry
  • NFI Transcription Factors
  • Nuclear Proteins
  • Transcription Factors/metabolism
  • Virus Replication
  • Y-Box-Binding Protein 1


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