Nuclear transfer in rodents

L J Mullins, I Wilmut, J J Mullins

Research output: Contribution to journalLiterature reviewpeer-review

Abstract / Description of output

Cloning is the asexual reproduction of an individual, such that the offspring have an essentially identical nuclear genome. Nuclear transfer and cloning have been achieved in a number of species, namely sheep, cows, goats, rabbits, cats and mice, but have been largely unsuccessful, so far, in dogs, primates and rats, Clearly, contributory factors which affect the outcome of successful cloning experiments are not universally applicable to all species. One theme common to all cloning experiments, however, is the overall inefficiency of the process, typically 0-4%. A number of factors contribute to nuclear transfer inefficiency, and we will review mouse cloning experiments, which address these problems, highlighting the importance of donor nucleus choice (somatic or ES cell, fetal or adult, quiescent or actively dividing). Finally, we will summarize the emerging principles which appear to govern nuclear reprogramming and production of clones, and will consider the application of nuclear transfer to the rat.

Original languageEnglish
Pages (from-to)4-12
Number of pages9
JournalThe Journal of Physiology
Volume554
Issue number1
DOIs
Publication statusPublished - 1 Jan 2004

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