Nucleolus organiser regions on mitotic and meiotic chromosomes from infertile males investigated using a specific silver stain

J Ironside, M J Faed

Research output: Contribution to journalArticlepeer-review

Abstract

Mitotic preparations from 30 subfertile males and meiotic preparations from 3 normal and 2 subfertile males were examined by means of the Ag-I technique of Bloom and Goodpasture (1976) to reveal nucleolus organiser regions (NORs). In the mitotic preparations, each subject was found to have a characteristic number of Ag-positive NORs per cell, within a range of 6--10. Analysis of satellite associations showed that the mean number of satellite associations per cell was related to the modal number of Ag-positive NORs for each subject. In the meiotic preparations, silver deposition was observed throughout meiotic prophase, but disappeared totally during diakinesis and metaphase II. It was seen again in early spermatids, and disappeared again as nuclear elongation took place. This pattern was observed in both normal and subfertile subjects, and may provide indirect evidence for the activation of rRNA genes during spermatogenesis.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalHuman Genetics
Volume48
Issue number1
Publication statusPublished - 17 Apr 1979

Keywords

  • Acrosome
  • Adult
  • Cell Nucleolus
  • Chromosomes
  • DNA
  • Humans
  • Infertility, Male
  • Male
  • Meiosis
  • Mitosis
  • Silver
  • Spermatogenesis
  • Staining and Labeling

Fingerprint

Dive into the research topics of 'Nucleolus organiser regions on mitotic and meiotic chromosomes from infertile males investigated using a specific silver stain'. Together they form a unique fingerprint.

Cite this