Nucleotide sequence of EV1, a British isolate of maedi-visna virus

David R. Sargan, Ian Bennet, Christina Cousens, Douglas J Roy, Barbara A. Blacklaws, Robert G. Dalziel, Neil J Watt, I. McConnell

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated a maedi-visna-like virus from the peripheral blood mononuclear cells of a British sheep displaying symptoms of arthritis and pneumonia. After brief passage in fibroblasts this virus (designated EV1) was used to infect choroid plexus cells. cDNA clones of the virus were prepared from these cells and sequenced. Gaps between non-overlapping clones were filled using gene amplification by the polymerase chain reaction. The genome structure is similar to that described for visna virus strain 1514, and differs from that described for visna virus strain SA-OMVV in not having a W reading frame. Overall the genome differs by about 20% between each of these strains, but there is fivefold variation in the amount of divergence of derived amino acid sequences of different open reading frames. Two sequenced EV1 clones each contain only one copy of the 43 bp repeat, with paired AP-1 sites, which is a feature of other ruminant lentiviral long terminal repeats (LTRs). However, analysis of viral DNA in infected cells by gene amplification shows that LTRs with two repeats do occur, albeit at a relatively low frequency.
Original languageEnglish
Pages (from-to)1893-903
Number of pages11
JournalJournal of General Virology
Volume72
Issue number8
DOIs
Publication statusPublished - Aug 1991

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Cells, Cultured
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Viral
  • Genes, Viral
  • Genes, env
  • Genes, gag
  • Genes, pol
  • Genetic Variation
  • Molecular Sequence Data
  • Open Reading Frames
  • Pneumonia, Progressive Interstitial, of Sheep/microbiology
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid
  • Sequence Alignment
  • Sheep
  • Visna-maedi virus/genetics
  • Visna-maedi virus & purification

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