Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer

Christopher C Thorn, Deborah Williams, Thomas C Freeman

Research output: Contribution to journalArticlepeer-review

Abstract

This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.
Original languageEnglish
Pages (from-to)203-21
Number of pages19
JournalMethods in Molecular Biology
Volume755
DOIs
Publication statusPublished - 2011

Keywords

  • Colorectal Neoplasms
  • DNA, Complementary
  • Data Interpretation, Statistical
  • Gene Expression Profiling
  • Humans
  • Lasers
  • Microdissection
  • Neoplasm Invasiveness
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Software
  • Staining and Labeling

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