Abstract
This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.
Original language | English |
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Pages (from-to) | 203-21 |
Number of pages | 19 |
Journal | Methods in Molecular Biology |
Volume | 755 |
DOIs | |
Publication status | Published - 2011 |
Keywords
- Colorectal Neoplasms
- DNA, Complementary
- Data Interpretation, Statistical
- Gene Expression Profiling
- Humans
- Lasers
- Microdissection
- Neoplasm Invasiveness
- Oligonucleotide Array Sequence Analysis
- Phenotype
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Software
- Staining and Labeling