Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer

Christopher C Thorn; Deborah Williams; Thomas C Freeman

doi:10.1007/978-1-61779-163-5_17

Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer

Christopher C Thorn, Deborah Williams, Thomas C Freeman

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.

Original language	English
Pages (from-to)	203-21
Number of pages	19
Journal	Methods in Molecular Biology
Volume	755
DOIs	https://doi.org/10.1007/978-1-61779-163-5_17
Publication status	Published - 2011

Keywords

Colorectal Neoplasms
DNA, Complementary
Data Interpretation, Statistical
Gene Expression Profiling
Humans
Lasers
Microdissection
Neoplasm Invasiveness
Oligonucleotide Array Sequence Analysis
Phenotype
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Software
Staining and Labeling

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10.1007/978-1-61779-163-5_17

Cite this

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title = "Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer",

abstract = "This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.",

keywords = "Colorectal Neoplasms, DNA, Complementary, Data Interpretation, Statistical, Gene Expression Profiling, Humans, Lasers, Microdissection, Neoplasm Invasiveness, Oligonucleotide Array Sequence Analysis, Phenotype, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Software, Staining and Labeling",

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AB - This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.

KW - Colorectal Neoplasms

KW - DNA, Complementary

KW - Data Interpretation, Statistical

KW - Gene Expression Profiling

KW - Humans

KW - Lasers

KW - Microdissection

KW - Neoplasm Invasiveness

KW - Oligonucleotide Array Sequence Analysis

KW - Phenotype

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Software

KW - Staining and Labeling

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DO - 10.1007/978-1-61779-163-5_17

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VL - 755

SP - 203

EP - 221

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer

Abstract

Keywords

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