On-line untargeted metabolomics monitoring of an escherichia coli succinate fermentation process

Joan Cortada-Garcia, Jennifer Haggarty, Tessa Moses, Rónán Daly, Susan Alison Arnold, Karl Burgess*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The real-time monitoring of metabolites (RTMet) is instrumental for the industrial production of biobased fermentation products. This study shows the first application of untargeted on-line metabolomics for the monitoring of undiluted fermentation broth samples taken automatically from a 5 L bioreactor every 5 min via flow injection mass spectrometry. The travel time from the bioreactor to the mass spectrometer was 30 s. Using mass spectrometry allows, on the one hand, the direct monitoring of targeted key process compounds of interest and, on the other hand, provides information on hundreds of additional untargeted compounds without requiring previous calibration data. In this study, this technology was applied in an Escherichia coli succinate fermentation process and 886 different m/z signals were monitored, including key process compounds (glucose, succinate, and pyruvate), potential biomarkers of biomass formation such as (R)-2,3-dihydroxy-isovalerate and (R)-2,3-dihydroxy-3-methylpentanoate and compounds from the pentose phosphate pathway and nucleotide metabolism, among others. The main advantage of the RTMet technology is that it allows the monitoring of hundreds of signals without the requirement of developing partial least squares regression models, making it a perfect tool for bioprocess monitoring and for testing many different strains and process conditions for bioprocess development.

Original languageEnglish
Pages (from-to)2757-2769
Number of pages13
JournalBiotechnology and Bioengineering
Issue number10
Early online date7 Jul 2022
Publication statusPublished - 1 Oct 2022

Keywords / Materials (for Non-textual outputs)

  • bioprocess monitoring
  • fermentation monitoring
  • on-line metabolomics
  • real-time metabolomics
  • succinate


Dive into the research topics of 'On-line untargeted metabolomics monitoring of an escherichia coli succinate fermentation process'. Together they form a unique fingerprint.

Cite this