One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins

Denis Lafontaine, David Tollervey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.

Original languageEnglish
Pages (from-to)3469-3471
Number of pages3
JournalNucleic Acids Research
Volume24
Issue number17
Publication statusPublished - 17 Sep 1996

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