Abstract
With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.
| Original language | English |
|---|---|
| Pages (from-to) | 3469-3471 |
| Number of pages | 3 |
| Journal | Nucleic Acids Research |
| Volume | 24 |
| Issue number | 17 |
| Publication status | Published - 17 Sept 1996 |