Operon gene order is optimized for ordered protein complex assembly

Jonathan N. Wells, L. Therese Bergendahl, Joseph A. Marsh

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The assembly of heteromeric protein complexes is an inherently stochastic process in which multiple genes are expressed separately into proteins, which must then somehow find each other within the cell. Here, we considered one of the ways by which prokaryotic organisms have attempted to maximize the efficiency of protein complex assembly: the organization of subunit-encoding genes into operons. Using structure-based assembly predictions, we show that operon gene order has been optimized to match the order in which protein subunits assemble. Exceptions to this are almost entirely highly expressed proteins for which assembly is less stochastic and for which precisely ordered translation offers less benefit. Overall, these results show that ordered protein complex assembly pathways are of significant biological importance and represent a major evolutionary constraint on operon gene organization.
Original languageEnglish
Pages (from-to)679–685
Number of pages7
JournalCell Reports
Volume14
Issue number4
Early online date21 Jan 2016
DOIs
Publication statusPublished - 2 Feb 2016

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