Opioid medication use and blood DNA methylation: epigenome-wide association meta-analysis

Mikyeong Lee, Roby Joehanes, Daniel L McCartney, Minjung Kho, Anke Hüls, Annah B Wyss, Chunyu Liu, Rosie M Walker, Sharon L R Kardia, Thomas S Wingo, Adam Burkholder, Jiantao Ma, Archie Campbell, Aliza P Wingo, Tianxiao Huan, Sinjini Sikdar, Amena Keshawarz, David A Bennett, Jennifer A Smith, Kathryn L EvansDaniel Levy, Stephanie J London

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Aim: To identify differential methylation related to prescribed opioid use. Methods: This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Results: Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: KIAA0226, CPLX2, TDRP, RNF38, TTC23 and GPR179. Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. Conclusion: This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.

Original languageEnglish
Early online date26 Jan 2023
Publication statusE-pub ahead of print - 26 Jan 2023


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