@article{8651d58096ef4adfab873f545904bbf8,
title = "Opioid medication use and blood DNA methylation: epigenome-wide association meta-analysis",
abstract = "Aim: To identify differential methylation related to prescribed opioid use. Methods: This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Results: Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: KIAA0226, CPLX2, TDRP, RNF38, TTC23 and GPR179. Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. Conclusion: This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.",
author = "Mikyeong Lee and Roby Joehanes and McCartney, {Daniel L} and Minjung Kho and Anke H{\"u}ls and Wyss, {Annah B} and Chunyu Liu and Walker, {Rosie M} and {R Kardia}, {Sharon L} and Wingo, {Thomas S} and Adam Burkholder and Jiantao Ma and Archie Campbell and Wingo, {Aliza P} and Tianxiao Huan and Sinjini Sikdar and Amena Keshawarz and Bennett, {David A} and Smith, {Jennifer A} and Evans, {Kathryn L} and Daniel Levy and London, {Stephanie J}",
note = "Funding Information: This work was supported in part by the Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Science (Z01-ES043012). Infrastructure for the CHARGE Consortium is supported in part by the National Heart, Lung, and Blood Institute grant HL105756. The Agricultural Lung Health Study is supported by the Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences (Z01-ES102385, Z01-ES049030, Z01-ES043012 and for ABW, contract no. HHSN273201600003I) and the National Cancer Institute (Z01-CP010119). This work was supported in part by American Recovery and Reinvestment Act (ARRA) funds through NIEHS contract N01-ES-55546. The Framingham Heart Study is funded by National Institutes of Health contract N01-HC-25195 and HHSN268201500001I. The laboratory work for this investigation was funded by the Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health. The analytical component of this project was funded by the Division of Intramural Research, National Heart, Lung, and Blood Institute, and the Center for Information Technology, National Institutes of Health, Bethesda, MD. Disclaimer: The views expressed in this manuscript are those of the authors and do not necessarily represent the views of the National Heart, Lung, and Blood Institute; the National Institutes of Health; or the U.S. Department of Health and Human Services. Generation Scotland received core support from the Chief Scientist Office of the Scottish Government Health Directorates (CZD/16/6) and the Scottish Funding Council (HR03006) and is currently supported by the Wellcome Trust (216767/Z/19/Z). Genotyping of the GS:SFHS samples was carried out by the Genetics Core Laboratory at the Edinburgh Clinical Research Facility, University of Edinburgh, Scotland, and was funded by the Medical Research Council UK and the Wellcome Trust (Wellcome Trust Strategic Award “STratifying Resilience and Depression Longitudinally” [STRADL] reference 104036/Z/14/Z). The DNA methylation profiling and analysis were supported by Wellcome Investigator Award 220857/Z/20/Z and grant 104036/Z/14/Z (PI: AM McIntosh) and through funding from NARSAD (ref: 27404; awardee: DM Howard) and the Royal College of Physicians of Edinburgh (Sim Fellowship; awardee: HC Whalley). Support for the Genetic Epidemiology Network of Arteriopathy was provided by the National Heart, Lung and Blood Institute (U01 HL054457, RC1 HL100185, R01 HL087660, R01 HL119443 and R01 HL133221). ROS/MAP is supported by P30AG10161, P30AG72975, R01AG15819, R01AG17917, U01AG46152, U01AG61356. ROS/MAP resources can be requested at https://www.radc.rush.edu . A H{\"u}ls is supported by the HERCULES Center (NIEHS P30ES019776). The authors declare that they have no relevant conflicts of interest. Publisher Copyright: {\textcopyright} 2023 National Institutes of Health.",
year = "2023",
month = jan,
day = "26",
doi = "10.2217/epi-2022-0353",
language = "English",
journal = "Epigenomics",
issn = "1750-192X",
publisher = "Future Medicine Ltd.",
}