Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

Tashfeen Aslam; Amy Miele; Sunay V Chankeshwara; Alicia Megia Fernandez; Chesney Michels; Ahsan R. Akram; Neil McDonald; Nik Hirani; Chris Haslett; Mark Bradley; Kevin Dhaliwal

doi:10.1039/c5sc01258a

Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

Tashfeen Aslam, Amy Miele, Sunay V Chankeshwara, Alicia Megia Fernandez, Chesney Michels, Ahsan R. Akram, Neil McDonald, Nik Hirani, Chris Haslett, Mark Bradley, Kevin Dhaliwal

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.

Original language	English
Pages (from-to)	4946-4953
Journal	Chemical Science
DOIs	https://doi.org/10.1039/c5sc01258a
Publication status	Published - 8 Jun 2015

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10.1039/c5sc01258a

C5SC01258A
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 1.02 MBLicence: Creative Commons: Attribution (CC-BY)

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M.
EPSRC
1/10/13 → 31/03/19
Project: Research
Molecular Optical Imaging of Key Targets in the Fibrogenic Pathway in Man
Bradley, M., Dhaliwal, K., Haslett, C., Hirani, N. & McGorum, B.
MRC
1/09/12 → 31/12/15
Project: Research

Kevin Dhaliwal
- Kev.Dhaliwaled.acuk
- Deanery of Clinical Sciences - Personal Chair of Molecular Imaging & Healthcare
- Centre for Inflammation Research
- Edinburgh Imaging
Person: Academic: Research Active , Academic: Research Active (Research Assistant)

Cite this

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title = "Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue",

abstract = "Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.",

author = "Tashfeen Aslam and Amy Miele and Chankeshwara, {Sunay V} and {Megia Fernandez}, Alicia and Chesney Michels and Akram, {Ahsan R.} and Neil McDonald and Nik Hirani and Chris Haslett and Mark Bradley and Kevin Dhaliwal",

year = "2015",

month = jun,

day = "8",

doi = "10.1039/c5sc01258a",

language = "English",

pages = "4946--4953",

journal = "Chemical Science",

issn = "2041-6539",

publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

AU - Aslam, Tashfeen

AU - Miele, Amy

AU - Chankeshwara, Sunay V

AU - Megia Fernandez, Alicia

AU - Michels, Chesney

AU - Akram, Ahsan R.

AU - McDonald, Neil

AU - Hirani, Nik

AU - Haslett, Chris

AU - Bradley, Mark

AU - Dhaliwal, Kevin

PY - 2015/6/8

Y1 - 2015/6/8

N2 - Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.

AB - Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.

U2 - 10.1039/c5sc01258a

DO - 10.1039/c5sc01258a

M3 - Article

SN - 2041-6539

SP - 4946

EP - 4953

JO - Chemical Science

JF - Chemical Science

ER -

Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

Abstract / Description of output

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Projects

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging

Molecular Optical Imaging of Key Targets in the Fibrogenic Pathway in Man

Profiles

Kevin Dhaliwal

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