Optical projection tomography as a tool for 3D microscopy and gene expression studies

James Sharpe; Ulf Ahlgren; Paul Perry; Bill Hill; Allyson Ross; Jacob Hecksher-Sørensen; Richard Baldock; Duncan Davidson

doi:10.1126/science.1068206

Optical projection tomography as a tool for 3D microscopy and gene expression studies

James Sharpe, Ulf Ahlgren, Paul Perry, Bill Hill, Allyson Ross, Jacob Hecksher-Sørensen, Richard Baldock, Duncan Davidson

Research output: Contribution to journal › Article › peer-review

Abstract

Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.

Original language	English
Pages (from-to)	541-5
Number of pages	5
Journal	Science
Volume	296
Issue number	5567
DOIs	https://doi.org/10.1126/science.1068206
Publication status	Published - 19 Apr 2002

Keywords

Anatomy, Cross-Sectional
Magnetic Resonance Imaging
Software
Animals
DNA-Binding Proteins
Hepatocyte Nuclear Factor 3-beta
Gene Expression
Nervous System
Mice
Microscopy, Fluorescence
Gene Expression Profiling
In Situ Hybridization
Transcription Factors
Nuclear Proteins
Embryonic and Fetal Development
Microscopy
Tomography
Mutation
Embryo, Mammalian
Fluorescent Antibody Technique
Image Processing, Computer-Assisted
Immunohistochemistry
Stomach
Neurofilament Proteins

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title = "Optical projection tomography as a tool for 3D microscopy and gene expression studies",

abstract = "Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by {"}optically sectioning{"} the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.",

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author = "James Sharpe and Ulf Ahlgren and Paul Perry and Bill Hill and Allyson Ross and Jacob Hecksher-S{\o}rensen and Richard Baldock and Duncan Davidson",

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T1 - Optical projection tomography as a tool for 3D microscopy and gene expression studies

AU - Sharpe, James

AU - Ahlgren, Ulf

AU - Perry, Paul

AU - Hill, Bill

AU - Ross, Allyson

AU - Hecksher-Sørensen, Jacob

AU - Baldock, Richard

AU - Davidson, Duncan

PY - 2002/4/19

Y1 - 2002/4/19

N2 - Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.

AB - Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.

KW - Anatomy, Cross-Sectional

KW - Magnetic Resonance Imaging

KW - Software

KW - Animals

KW - DNA-Binding Proteins

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KW - Gene Expression

KW - Nervous System

KW - Mice

KW - Microscopy, Fluorescence

KW - Gene Expression Profiling

KW - In Situ Hybridization

KW - Transcription Factors

KW - Nuclear Proteins

KW - Embryonic and Fetal Development

KW - Microscopy

KW - Tomography

KW - Mutation

KW - Embryo, Mammalian

KW - Fluorescent Antibody Technique

KW - Image Processing, Computer-Assisted

KW - Immunohistochemistry

KW - Stomach

KW - Neurofilament Proteins

U2 - 10.1126/science.1068206

DO - 10.1126/science.1068206

M3 - Article

C2 - 11964482

VL - 296

SP - 541

EP - 545

JO - Science

JF - Science

SN - 0036-8075

IS - 5567

ER -

Optical projection tomography as a tool for 3D microscopy and gene expression studies

Abstract

Keywords

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