Projects per year
Abstract / Description of output
Improving the speed and accuracy of bacterial detection is important for patient stratification and to ensure the appropriate use of antimicrobials. To achieve this goal, the development of diagnostic techniques to recognize bacterial presence in real-time at the point-of-care is required. Optical imaging for direct identification of bacteria within the host is an attractive approach. Several attempts at chemical probe design and validation have been investigated, however none have yet been successfully translated into the clinic. Here we describe a method for ex vivo validation of bacteria-specific probes for identification of bacteria within the distal lung, imaged by fibered confocal fluorescence microscopy (FCFM). Our model used ex vivo human lung tissue and a clinically approved confocal laser endomicroscopy (CLE) platform to screen novel bacteria-specific imaging compounds, closely mimicking imaging conditions expected to be encountered with patients. Therefore, screening compounds by this technique provides confidence of potential clinical tractability.
Original language | English |
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Journal | Journal of Visualized Experiments (JoVE) |
Early online date | 13 Aug 2017 |
DOIs | |
Publication status | E-pub ahead of print - 13 Aug 2017 |
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Dive into the research topics of 'Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy'. Together they form a unique fingerprint.Projects
- 2 Finished
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Proteus Participation in CARB-X
Walsh, T., Dhaliwal, K. & Haslett, C.
1/04/17 → 31/07/19
Project: Research
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Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M.
1/10/13 → 31/03/19
Project: Research