Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy

Mathieu A. Bennet, Patricia R. Richardson, Jochen Arlt, Aongus McCarthy, Gerald S. Buller, Anita C. Jones

Research output: Contribution to journalArticlepeer-review

Abstract

The novel combination of optical tweezers and fluorescence lifetime imaging microscopy (FLIM) has been used, in conjunction with specially developed temperature-sensitive fluorescent microprobes, for the non-invasive measurement of temperature in a microfluidic device. This approach retains the capability of FLIM to deliver quantitative mapping of microfluidic temperature without the disadvantageous need to introduce a fluorescent dye that pervades the entire micofluidic system. This is achieved by encapsulating the temperature-sensitive Rhodamine B fluorophore within a microdroplet which can be held and manipulated in the microfluidic flow using optical tweezers. The microdroplet is a double bubble in which an aqueous droplet of the fluorescent dye is surrounded by an oil shell which serves both to contain the fluorophore and to provide the refractive index differential required for optical trapping of the droplet in an external aqueous medium.

Original languageEnglish
Pages (from-to)3821-3828
Number of pages8
JournalLab on a Chip
Volume11
Issue number22
Early online date28 Sep 2011
DOIs
Publication statusPublished - 21 Nov 2011

Keywords

  • RHODAMINE-B
  • TWEEZERS
  • FLOW
  • CELL
  • SYSTEM
  • ACQUISITION
  • RESOLUTION
  • TRANSPORT
  • EVOLUTION
  • GRADIENT

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