Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence

D A Hume; S Gordon

doi:10.1002/jcp.1041170209

Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence

D A Hume, S Gordon

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.

Original language	English
Pages (from-to)	189-94
Number of pages	6
Journal	Journal of Cellular Physiology
Volume	117
Issue number	2
DOIs	https://doi.org/10.1002/jcp.1041170209
Publication status	Published - Nov 1983

Keywords / Materials (for Non-textual outputs)

Animals
Blood
Bone Marrow
Calcium
Cell Adhesion
Cells, Cultured
Colony-Stimulating Factors
Culture Media
L Cells (Cell Line)
Macrophages
Mice

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10.1002/jcp.1041170209

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title = "Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence",

abstract = "A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.",

keywords = "Animals, Blood, Bone Marrow, Calcium, Cell Adhesion, Cells, Cultured, Colony-Stimulating Factors, Culture Media, L Cells (Cell Line), Macrophages, Mice",

author = "Hume, {D A} and S Gordon",

year = "1983",

month = nov,

doi = "10.1002/jcp.1041170209",

language = "English",

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journal = "Journal of Cellular Physiology",

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T1 - Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture

T2 - the roles of CSF-1, serum, Ca2+, and adherence

AU - Hume, D A

AU - Gordon, S

PY - 1983/11

Y1 - 1983/11

N2 - A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.

AB - A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.

KW - Animals

KW - Blood

KW - Bone Marrow

KW - Calcium

KW - Cell Adhesion

KW - Cells, Cultured

KW - Colony-Stimulating Factors

KW - Culture Media

KW - L Cells (Cell Line)

KW - Macrophages

KW - Mice

U2 - 10.1002/jcp.1041170209

DO - 10.1002/jcp.1041170209

M3 - Article

C2 - 6605349

SN - 0021-9541

VL - 117

SP - 189

EP - 194

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 2

ER -

Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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