Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence

D A Hume, S Gordon

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.
Original languageEnglish
Pages (from-to)189-94
Number of pages6
JournalJournal of Cellular Physiology
Volume117
Issue number2
DOIs
Publication statusPublished - Nov 1983

Keywords

  • Animals
  • Blood
  • Bone Marrow
  • Calcium
  • Cell Adhesion
  • Cells, Cultured
  • Colony-Stimulating Factors
  • Culture Media
  • L Cells (Cell Line)
  • Macrophages
  • Mice

Fingerprint

Dive into the research topics of 'Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence'. Together they form a unique fingerprint.

Cite this