Optimisation of analyte transport in integrated microfluidic affinity sensors for the quantification of low levels of analyte

Daniel Friedrich, Colin P Please, Tracy Melvin

Research output: Contribution to journalArticlepeer-review

Abstract

New designs for microfluidic channels to be integrated with small-scale affinity sensors for analytical applications are provided. Theoretical approaches demonstrate efficient and uniform mass transfer of the analyte from the bulk flow to small-scale affinity sensors in the base of fluidic channels by (i) active control of the analyte flow speed over the affinity sensor, (ii) non-rectangular channel geometries and (iii) non-uniform distributions of recognition binding sites over the active area of the sensor. The methodology reported provides generic strategies that can be exploited for small-scale sensors in single or multiplex formats.
Original languageEnglish
Pages (from-to)323-332
JournalSensors and Actuators B: Chemical
Volume131
Issue number1
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Affinity sensor
  • Microfluidics
  • Mass transfer
  • Laminar flow
  • Biosensor
  • Fluidic channel
  • Detection
  • Design optimisation
  • Recognition molecule
  • Analyte depletion

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