Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Abigail Jeorrett, Steven L. Neale, David Massoubre, Erdan Gu, Robert Henderson, O R Millington, Keith Mathieson, Martin D. Dawson

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.
Original languageEnglish
Pages (from-to)1372-1380
Number of pages8
JournalOptics Express
Volume22
Issue number2
DOIs
Publication statusPublished - 14 Jan 2014

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