Orientation specificity of the Lactococcus lactis Chi site

M El Karoui, M Schaeffer, V Biaudet, A Bolotin, A Sorokin, A Gruss

Research output: Contribution to journalArticlepeer-review


In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. ChiEc is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA break repair. We previously identified a Chi-like sequence (referred to as ChiLl) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. ChiLl and RexAB are functional analogues of ChiEc and RecBCD.
Original languageEnglish
Pages (from-to)453-61
Number of pages9
JournalGenes to Cells
Issue number6
Publication statusPublished - 2000


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