Orientation specificity of the Lactococcus lactis Chi site

M El Karoui; M Schaeffer; V Biaudet; A Bolotin; A Sorokin; A Gruss

doi:10.1046/j.1365-2443.2000.00342.x

Orientation specificity of the Lactococcus lactis Chi site

M El Karoui, M Schaeffer, V Biaudet, A Bolotin, A Sorokin, A Gruss

Research output: Contribution to journal › Article › peer-review

Abstract

In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. ChiEc is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA break repair. We previously identified a Chi-like sequence (referred to as ChiLl) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. ChiLl and RexAB are functional analogues of ChiEc and RecBCD.

Original language	English
Pages (from-to)	453-61
Number of pages	9
Journal	Genes to Cells
Volume	5
Issue number	6
DOIs	https://doi.org/10.1046/j.1365-2443.2000.00342.x
Publication status	Published - 2000

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title = "Orientation specificity of the Lactococcus lactis Chi site",

abstract = "In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. ChiEc is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA break repair. We previously identified a Chi-like sequence (referred to as ChiLl) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. ChiLl and RexAB are functional analogues of ChiEc and RecBCD.",

author = "{El Karoui}, M and M Schaeffer and V Biaudet and A Bolotin and A Sorokin and A Gruss",

year = "2000",

doi = "10.1046/j.1365-2443.2000.00342.x",

language = "English",

volume = "5",

pages = "453--61",

journal = "Genes to Cells",

issn = "1356-9597",

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TY - JOUR

T1 - Orientation specificity of the Lactococcus lactis Chi site

AU - El Karoui, M

AU - Schaeffer, M

AU - Biaudet, V

AU - Bolotin, A

AU - Sorokin, A

AU - Gruss, A

PY - 2000

Y1 - 2000

N2 - In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. ChiEc is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA break repair. We previously identified a Chi-like sequence (referred to as ChiLl) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. ChiLl and RexAB are functional analogues of ChiEc and RecBCD.

AB - In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. ChiEc is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA break repair. We previously identified a Chi-like sequence (referred to as ChiLl) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. ChiLl and RexAB are functional analogues of ChiEc and RecBCD.

U2 - 10.1046/j.1365-2443.2000.00342.x

DO - 10.1046/j.1365-2443.2000.00342.x

M3 - Article

C2 - 10886371

VL - 5

SP - 453

EP - 461

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 6

ER -

Orientation specificity of the Lactococcus lactis Chi site

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