TY - JOUR
T1 - Osteoclast formation in mouse co-cultures
AU - Itzstein, Cecile
AU - van 't Hof, Robert J
PY - 2012
Y1 - 2012
N2 - The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D(3) and prostaglandin E(2). This assay is used particularly to analyse osteoblast-osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.
AB - The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D(3) and prostaglandin E(2). This assay is used particularly to analyse osteoblast-osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.
UR - http://www.scopus.com/inward/record.url?scp=84555195955&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-415-5_12
DO - 10.1007/978-1-61779-415-5_12
M3 - Article
C2 - 22130929
VL - 816
SP - 177
EP - 186
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -