Osteoclast formation in mouse co-cultures

Cecile Itzstein, Robert J van 't Hof

Research output: Contribution to journalArticlepeer-review


The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D(3) and prostaglandin E(2). This assay is used particularly to analyse osteoblast-osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.
Original languageEnglish
Pages (from-to)177-86
Number of pages10
JournalMethods in Molecular Biology
Publication statusPublished - 2012


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