Ovation RRBS Methyl-seq library preparation protocol

This protocol describes a library prep procedure for 1 to 96 samples with the Ovation RRBS Methyl-seq System. The original Tecan Ovation protocol describes a low throughput procedure, therefore this protocol is especially targeted for manual high-throughput preps from 24 to 96 samples, although it can be used for small sample numbers too. Importantly, the Ovation RRBS kit is currently sold as oxBS only (identifying both 5-methyl-C and 5-hydroxymethyl-C), whilst this protocol is modified to exclude the oxidation step and describes a basic RRBS, where both 5mC and 5hmC appear as the same positive signal and are indistinguishable. This protocol has a few further modifications, specifically in the bisulfite conversion step and bead purifications, which have performed better in our hands.