Over expression of IL-10 by macrophages overcomes resistance to murine filariasis

Sabine Specht, Matthew D Taylor, Marieke Hoeve, Judith E Allen, Roland Lang, Achim Hoerauf

Research output: Contribution to journalArticlepeer-review

Abstract

Individuals infected with parasitic helminths are able to tolerate the presence of parasites for considerable time without clinical pathology. Immunosuppressive responses induced by the filarial parasite are considered responsible for this long-lasting relationship, inuring to the benefit of both parasite and host. In order to directly link IL-10 with parasite survival, we infected mice, in which over expression of IL-10 was restricted to macrophages under control of the CD68 promoter (macIL-10tg), with Litomosoides sigmodontis. IL-10 overexpression by macrophages led to increased susceptibility with a significantly higher number of adult worms. Most profound, IL-10 overexpression was sufficient to convert resistant FVB wild-type mice towards a patent phenotype, since microfilariae were exclusively found in macIL-10tg mice. These findings were associated with reduced Th2 cytokine production in macIL-10tg mice. Expression of arginase-1, Ym1 and Fizz1, genes that are found strongly expressed in murine alternatively activated macrophages, were detected in macIL-10tg mice. Thus, IL-10 produced by macrophages with characteristics of alternative activation can overcome resistance and allow full patency in murine filariasis.
Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalExperimental Parasitology
Volume132
Issue number1
Early online date19 Sep 2011
DOIs
Publication statusPublished - 30 Sep 2012

Keywords

  • Murine filariasis
  • IL-10
  • Immunosuppression
  • Microfilaria
  • Patency

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