Overexpression of the single-stranded DNA-binding protein (SSB) stabilises CAG center dot CTG triplet repeats in an orientation dependent manner

Federica Andreoni, Elise Darmon, Wilson C. K. Poon, David R. F. Leach

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The stability and deletion-size-distribution profiles of leading strand (CAG)(75) and (CTG)(137) trinucleotide repeat arrays inserted in the Escherichia coli chromosome were investigated upon overexpression of the single-stranded DNA-binding protein (SSB) and in mutant strains deficient for the SbcCD (Rad51/Mre11) nuclease. SSB overexpression increases the stability of the (CAG)(75) repeat array and leads to a loss of the bias towards large deletions for the same array. Furthermore, the absence of SbcCD leads to a reduction in the number of large deletions in strains containing the (CTG)(137) repeat array. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalFEBS Letters
Volume584
Issue number1
DOIs
Publication statusPublished - 4 Jan 2010

Keywords / Materials (for Non-textual outputs)

  • Trinucleotide repeat instability
  • Single-stranded DNA-binding protein
  • SbcCD
  • HUMAN GENETIC-DISEASE
  • ESCHERICHIA-COLI
  • TRINUCLEOTIDE REPEATS
  • DYNAMIC MUTATIONS
  • INSTABILITY
  • MECHANISMS
  • REPLICATION
  • CHROMOSOME
  • PALINDROME
  • EXPANSION

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