Oviduct-specific expression of two therapeutic proteins in transgenic hens

S G Lillico, A Sherman, M J McGrew, C D Robertson, J Smith, C Haslam, P Barnard, P A Radcliffe, K A Mitrophanous, E A Elliot, H M Sang

Research output: Contribution to journalArticlepeer-review

Abstract

Recent advances in avian transgenesis have led to the possibility of utilizing the laying hen as a production platform for the large-scale synthesis of pharmaceutical proteins. Ovalbumin constitutes more than half of the protein in the white of a laid egg, and expression of the ovalbumin gene is restricted to the tubular gland cells of the oviduct. Here we describe the use of lentiviral vectors to deliver transgene constructs comprising regulatory sequences from the ovalbumin gene designed to direct synthesis of associated therapeutic proteins to the oviduct. We report the generation of transgenic hens that synthesize functional recombinant pharmaceutical protein in a tightly regulated tissue-specific manner, without any evidence of transgene silencing after germ-line transmission.
Original languageEnglish
Pages (from-to)1771-6
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume104
Issue number6
DOIs
Publication statusPublished - 6 Feb 2007

Keywords

  • Animals
  • Animals, Genetically Modified
  • Chickens
  • Egg Proteins
  • Egg White
  • Female
  • Genetic Vectors
  • Humans
  • Infectious Anemia Virus, Equine
  • Interferon-beta
  • Male
  • Mice
  • Oviducts
  • Recombinant Proteins

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