p21-LacZ reporter mice reflect p53-dependent toxic insult

D. B. Vasey; C. R. Wolf; T. MacArtney; K. Brown; C. B. Whitelaw

doi:10.1016/j.taap.2007.11.029

p21-LacZ reporter mice reflect p53-dependent toxic insult

D. B. Vasey, C. R. Wolf, T. MacArtney, K. Brown, C. B. Whitelaw

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity.

Original language	English
Pages (from-to)	440-450
Number of pages	11
Journal	Toxicology and Applied Pharmacology
Volume	227
DOIs	https://doi.org/10.1016/j.taap.2007.11.029
Publication status	Published - Mar 2008

Keywords / Materials (for Non-textual outputs)

Cell cycle
Drug
Etoposide
Hippocampus
Toxicity
Transgenic mice

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10.1016/j.taap.2007.11.029

http://www.sciencedirect.com/science/article/pii/S0041008X07005224

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title = "p21-LacZ reporter mice reflect p53-dependent toxic insult",

abstract = "There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity.",

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T1 - p21-LacZ reporter mice reflect p53-dependent toxic insult

AU - Vasey, D. B.

AU - Wolf, C. R.

AU - MacArtney, T.

AU - Brown, K.

AU - Whitelaw, C. B.

PY - 2008/3

Y1 - 2008/3

N2 - There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity.

AB - There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity.

KW - Cell cycle, drug, etoposide, tixicity, transgenic mouse

KW - Cell cycle

KW - Drug

KW - Etoposide

KW - Hippocampus

KW - Toxicity

KW - Transgenic mice

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DO - 10.1016/j.taap.2007.11.029

M3 - Article

SN - 0041-008X

VL - 227

SP - 440

EP - 450

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

ER -

p21-LacZ reporter mice reflect p53-dependent toxic insult

Abstract

Keywords / Materials (for Non-textual outputs)

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