p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

A. Capocefalo, V. Franceschi, C. B. A. Whitelaw, D. B. Vasey, S. G. Lillico, S. Cavirani, G. Donofrio

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BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage. (C) 2009 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)308-311
Number of pages4
JournalJournal of Virological Methods
Issue number2
Publication statusPublished - Nov 2009

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