p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

A. Capocefalo; V. Franceschi; C. B. A. Whitelaw; D. B. Vasey; S. G. Lillico; S. Cavirani; G. Donofrio

doi:10.1016/j.jviromet.2009.06.012

p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

A. Capocefalo, V. Franceschi, C. B. A. Whitelaw, D. B. Vasey, S. G. Lillico, S. Cavirani, G. Donofrio

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage. (C) 2009 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	308-311
Number of pages	4
Journal	Journal of Virological Methods
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2009.06.012
Publication status	Published - Nov 2009

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title = "p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication",

abstract = "BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage. (C) 2009 Elsevier B.V. All rights reserved.",

author = "A. Capocefalo and V. Franceschi and Whitelaw, {C. B. A.} and Vasey, {D. B.} and Lillico, {S. G.} and S. Cavirani and G. Donofrio",

year = "2009",

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doi = "10.1016/j.jviromet.2009.06.012",

language = "English",

volume = "161",

pages = "308--311",

journal = "Journal of Virological Methods",

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TY - JOUR

T1 - p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

AU - Capocefalo, A.

AU - Franceschi, V.

AU - Whitelaw, C. B. A.

AU - Vasey, D. B.

AU - Lillico, S. G.

AU - Cavirani, S.

AU - Donofrio, G.

PY - 2009/11

Y1 - 2009/11

N2 - BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage. (C) 2009 Elsevier B.V. All rights reserved.

AB - BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage. (C) 2009 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.jviromet.2009.06.012

DO - 10.1016/j.jviromet.2009.06.012

M3 - Article

SN - 0166-0934

VL - 161

SP - 308

EP - 311

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

Abstract

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