p53-independent DNA repair and cell cycle arrest in embryonic stem cells

S Prost, Christopher Bellamy, A R Clarke, A H Wyllie, D J Harrison

Research output: Contribution to journalArticlepeer-review

Abstract

The role of p53 in DNA repair and cell cycle checkpoint after ultraviolet irradiation was investigated in an embryonic stem cell line homozygous for a targeted deletion of p53, Results indicate that loss of p53 does not alter the capacity of ES cells to respond to DNA damage. wild-type and p53-deficient cells showed similar cessation of DNA synthesis after UV damage and similar ultimate capacity to repair a transiently transfected reporter plasmid, Interestingly, in the absence of DNA damaging treatment, the transit of p53-deficient cells through S phase mas slower than wild-type cells. We suggest that this may result from the absence of a p53-dependent response to endogenous DNA damage: without p53 sensing endogenous damage leading to immediate repair, such damage may persist and thus delay DNA synthesis. (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalFEBS Letters
Volume425
Issue number3
DOIs
Publication statusPublished - 3 Apr 1998

Keywords

  • embryonic stem cell
  • p53
  • cell cycle
  • DNA repair
  • UV
  • DNA damage
  • LI-FRAUMENI SYNDROME
  • P53-DEFICIENT MOUSE CELLS
  • WILD-TYPE
  • ULTRAVIOLET-LIGHT
  • EXCISION-REPAIR
  • P53 MUTATIONS
  • DAMAGE
  • MICE
  • APOPTOSIS

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